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Description
For immunohistochemistry (IHC) labeling, heat-mediated epitope retrieval was performed in 0.01 m sodium citrate buffer, pH 6, containing 0.05% Tween-20, which was heated in a microwave oven to 95C for 30 min
Immunohistochemistry Sections were deparaffinized and rehydrated in xylene and ethanol
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Subsequent analysis indicated elevated expression of COX2 and -SMA in the fibrotic group, as observed through immunofluorescence staining (Fig
